C (3-6 months) INSERM INSERM <a href='https://brc.riken.jp/mus/pcr01678'>Genotyping protocol -PCR-</a> Neurobiology Research NF2KO3 NF2KO3 FVB.129P2-Nf2<tm1Gth>/GthRbrc FVB.129P2-Nf2<tm1Gth>/GthRbrc true Fluorescent Proteins/lacZ System The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes & Development., 14, 1617-1630 (2000).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT shall keep DEPOSITOR apprised of any results obtained from the BIOLOGICAL RESOURCE in the course of the research, as soon as they are available. Moreover, if the research results in a potential invention or a substance that may have potential commercial value (hereinafter referred as to the "Invention") RECIPIENT will promptly disclose this Invention to DEPOSITOR with samples thereof for DEPOSITOR's internal use.<br>RECIPIENT and DEPOSITOR will consult together and agree about the possibility of filing one or more patent application to Invention conceived by one or more employees of Recipients that directly arise out of the research. The Parties will negotiate in good faith the conditions of such patent application according to each party's inventiveness contribution in the Invention.<br>In case of such Invention, patentable or not, reduced in practice or developed in the course of the research that could not have been obtained, reduced to practice or developed but from using the Material and/or Confidential Information, RECIPIENT and DEPOSITOR shall confer with one another as regards licensing and commercialization of such Invention to negotiate in good faith the terms of an institutional agreement as regards said matters. Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, mouse Pgk promoter, E. coli Hyg, rabbit bata globin poly A Nf2 isoform 1 knock-out mice. Nf2, Neurofiboromatosis type 2 is a tumor suppressor gene encoding a putative cytoskeletal asssociated protein. Homozygous mutant mice die prior to E9.5. 44% of heterozygotes develop osteoblastic hyperplasia. 38% of heterozygotes exhibit renal tubular cell hyperplasia. 6% of heterozygotes exhibit odontoblastic hyperplasia. Developed by Drs. Marco Giovannini and Gilles Thomas, INSERM in 2000. A Ires-lacZ-hygromtcin resistance cassette was inserted into exon 3 of Nf2 gene. The mutant mice were backcrossed to FVB/N for 18 times. Marco Giovannini Marco Giovannini C（3〜6か月） Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Genes & Development., 14, 1617-1630 (2000).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>RECIPIENT shall keep DEPOSITOR apprised of any results obtained from the BIOLOGICAL RESOURCE in the course of the research, as soon as they are available. Moreover, if the research results in a potential invention or a substance that may have potential commercial value (hereinafter referred as to the "Invention") RECIPIENT will promptly disclose this Invention to DEPOSITOR with samples thereof for DEPOSITOR's internal use.<br>RECIPIENT and DEPOSITOR will consult together and agree about the possibility of filing one or more patent application to Invention conceived by one or more employees of Recipients that directly arise out of the research. The Parties will negotiate in good faith the conditions of such patent application according to each party's inventiveness contribution in the Invention.<br>In case of such Invention, patentable or not, reduced in practice or developed in the course of the research that could not have been obtained, reduced to practice or developed but from using the Material and/or Confidential Information, RECIPIENT and DEPOSITOR shall confer with one another as regards licensing and commercialization of such Invention to negotiate in good faith the terms of an institutional agreement as regards said matters. RBRC01678 アスベストで引き起こされるMalignant Mesotheliomasの半数にこの遺伝子のmutationが見られる。Nf2 KO +/-マウスにアスベストを暴露すると高率でLOHが起こり、中皮腫を引き起こす。